ABSTRACT
Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and three-dimensional reconstructions provide a valuable tool for revealing aspects of the synaptic morphology that are not available when conventional electron microscopy is used. By applying this selective staining technique and three-dimensional imaging, we uncovered the structural organization of actin in the postsynaptic densities in physiological and pathological conditions.
Subject(s)
Humans , Animals , Actins/metabolism , Eosine Yellowish-(YS)/pharmacology , Eosine Yellowish-(YS)/metabolism , Photooxidation , Central Nervous System/metabolism , Central Nervous System/ultrastructure , Staining and Labeling/methods , Fluorescent Dyes/pharmacology , Phalloidine/pharmacology , Imaging, Three-Dimensional/methods , Models, Molecular , Actin Cytoskeleton/metabolism , Actin Cytoskeleton/ultrastructure , Microscopy, Fluorescence/methods , Oxidation-Reduction , PhotonsABSTRACT
Se presenta un caso de enfermedad de Mondor de localizacion abdominal (flanco izquierdo), atribuible a la realizacion de repetidos movimientos musculares de hiperextension. El proceso asentaba en territorio de la vena toracoepigastrica en su sector abdominal, por debajo del reborde costal. Los autores analizan la bibliografia referente al tema, pasando revista a los sintomas, caracteristicas semiologicas e histopatologicas, posibles etiologias y diagnosticos diferenciales
Subject(s)
PhlebitisABSTRACT
Se presentan cinco casos de loxoscelismo cutaneo-necrotico simple, efectuandose consideraciones sobre diferentes aspectos historicos, etiopatogenicos, clinicos y terapeuticos de la enfermedad
Subject(s)
Spider Bites , Spider Venoms , Edema , NecrosisABSTRACT
Se presenta un paciente de 32 anos, del sexo masculino, con maculas eritematosas, algunas de las cuales se acompanan de papulas y vesiculas. Las lesiones producen ardor y prurito intensos, y localizan en region lumbar y hemitorax izquierdos, brazo y axila del mismo lado. En los primeros momentos presento ademas escalofrios y fiebre. Se verifica el contacto con un ejemplar hembra de Hylesia nigricans Berg, mariposa perteneciente a la familia Hemileucidae. Se analiza la etiopatogenia y se enumeran diversas alternativas en que dicho insecto, direta o indirectamente, en sus distintos estadios evolutivos, es capaz de provocar esta dermatitis